Chemo-enzymatic synthesis of nod factors; bacterial signaling molecules
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Maolanon, Nicolai Nareth1, Forfatter
Jensen, Knud J.1, Vejleder
K. Sørensen, Kasper1, Vejleder
Sauer, Jørgen1, Vejleder
Blais, Mickael1, Vejleder
Boesen, Thomas1, Vejleder
1Det Biovidenskabelige Fakultet, København, Danmark, diskurs:18              
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 Abstract: The symbiosis between legume plants and Rhizobium bacteria is important for optimal growthand development. Legumes include many food and feed products, therefore, optimal growth isof economical importance. The symbiotic relationship begins with Rhizobia entering the plantroots to form a new plant organ; the root nodule. In the nodule, Rhizobia xates theatmospheric nitrogen that is taken up by the plant. The conversion into ammonia allows theplant to use this nitrogen source in its metabolic processes. Nod factors are lipochitinoligosaccharides. These are the bacterial signaling molecules that are thought to be responsiblefor the specic recognition between the legume and Rhizobia and to initiate nodule formation.To obtain a highly pure nod factor in fair amounts, a chemo-enzymatic approach wasencountered. Deacetylation at the reducing end of N-pentaacetylchitopentaose was doneenzymatically using the enzyme NodB leaving one free amino group, while lipidation was doneby chemical means. Following this strategy 9 mg of nod factor, corresponding to 69% yield,were obtained and may be used in in vivo plant signaling experiments as well ascrystallography studies with the putative nod factor receptor. Although the synthesis of severalclosely related nod factors has been described, this exact structure has not yet been reported.
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 Type: Speciale
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Sprog: English - eng
 Datoer: 2008
 Sider: 114 pages
 Publiceringsinfo: København, Danmark : Institut for Grundvidenskab og Miljø
 Indholdsfortegnelse: -
 Note: Biology - Biotechnology, Biologi - Bioteknologi
 Type: Speciale
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